SPIDER - UNIX  - Chimera - 2D Image Processing - 3D Reconstruction

3D Image Reconstruction

I worked at Dr. Radermacher's Lab part time from March to June 2014. At the time I had an internship position  at the Instrumentation Facility and Technical Services Partnership (TSP) at the University of Vermont in Clinical Engineering with Prof. Tobey Clark. 

After work hours at my primary internship, I worked at Dr. Radermacher's Lab to learn more about image reconstruction techniques from 3D electron micrographs of macromolecular assemblies. The image you see to the right is one reconstruction I made from a random conical tilt series of Complex I from the Mitochondria!

 

Advisor: Michael Radermacher, PhD

 

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